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High fat diet mouse obesity surgery – Women: Lower-fat Diet Key to Liver Health Following Weight-loss Surgery

Int J Impot Res. Figure 1.

David Stewart
Wednesday, November 8, 2017
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  • Publication types Research Support, Non-U. Mokadem, M.

  • Exogenous peptide YY and Exendin-4 further decrease food intake, whereas octreotide increases food intake in rats after Roux-en-Y gastric bypass.

  • Roux-en-Y gastric bypass surgery is effective in fibroblast growth factor deficient mice.

  • In vitro electrically evoked tachykininergic contractions were enhanced in HFD mice after 2 or 8 weeks, and they were blunted by FC.

  • Roux-en-Y gastric bypass enhances energy expenditure and extends lifespan in diet-induced obese rats. Force, U.

MeSH terms

This is surprizing. Thus, these survery were completely protected from weight gain and metabolic disease for the equivalent of roughly half the typical human lifespan. Timing of bariatric surgery for severely obese adolescents: a Markov decision-analysis. Nat Rev Endocrinol: Beyond intestinal soap-bile acids in metabolic control; However, such a feature is well known during caloric restriction aimed to weight loss.

Obesity Silver Spring ; 19 — Sign up for email alerts. Antiobesity and emetic effects of a short-length peptide YY analog and its PEGylated and alkylated derivatives. Body weight was measured before and after surgery every 2—3 days for a total of 20 weeks when the animals were euthanized and tissues harvested. Furthermore, the 1. The research team found both male and female mice had better glucose tolerance and lost weight and body fat after surgery, even if they stayed on the high-fat diet. Y5 receptor signalling counteracts the anorectic effects of PYY in diet-induced obese mice.

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Diet and gastrointestinal bypass-induced weight loss: The roles of ghrelin and peptide YY. Glucose and Insulin Tolerance Tests Intraperitoneal glucose tolerance tests were performed at either month 3 or 4 in all cohorts, as indicated. Lymphoedema was followed over a period of 6 weeks measuring oedema, evaluating tissue histology and lymphatic vascular function. The gut hormone response following Roux-en-Y gastric bypass: cross-sectional and prospective study. After 12 weeks, ex vivo vascular reactivity was measured in aortas, internal pudendal arteries, and penises. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. These data indicate that plasma LEAP2 decreases in humans after 2 different types of weight loss surgery.

ME, median eminence. Effect of roux-en-Y gastric bypass surgery on bile acid metabolism in normal and obese diabetic rats. Figure 3. Results 3.

Associated Data

Comment about intestinal gluconeogenesis after gastric bypass in human in relation with the mpuse by Hayes et al. Effect obesity surgery roux-en-Y gastric bypass surgery on bile acid metabolism in normal and obese diabetic rats. Physiological modulation of intestinal motility by enteric dopaminergic neurons and the D2 receptor: analysis of dopamine receptor expression, location, development, and function in wild-type and knock-out mice. Melanocortin-4 receptor signaling is required for weight loss after gastric bypass surgery.

Together, hiyh results suggest a model Figure 8 in which, during negative energy balance acute fasting or longer-term energy restrictiona fall in plasma LEAP2 creates a permissive environment for elevated plasma acyl-ghrelin to most effectively act to increase food intake and GH secretion and prevent potentially life-threatening falls in blood glucose. Mouse studies. Brain Res. Aroda V. All data were analyzed using GraphPad Prism software version 7.

Glucose deriving from IGN is released petbox acquired hypothyroidism the portal vein and sensed by a portal glucose sensor, which initiates a gut-brain-liver neural circuit inducing satiety, an increase in hepatic insulin sensitivity and a decrease in hepatic glucose production [for a review, see [ 25 ]]. Statistical analysis Statistical analysis was performed using RStudio version 1. Skip to main content Thank you for visiting nature. Hofmann, B. However the potential contribution of differences in fecal energy loss needs further investigation.

Publication types

Schauer, P. Using a brain matrix Braintree Scientific, Braintree, MAa 1 mm coronal section was obtained and the mediobasal hypothalamus removed under dissecting microscope. In addition, the serious psychological problems associated with stigmatization and bullying drastically reduce quality of life and life expectancy 7.

  • References 1.

  • LEAP2 is synthesized as a 77—amino acid prohormone in humans 76 amino acids in mice 42 that subsequently is processed to its mature form consisting of 40 amino acids with 2 disulfide bridges spanning 4 highly conserved cysteine residues 41 ,

  • Unpaired Student's t test was used for two-group comparisons. Immonen H.

  • Appetite and body weight regulation after bariatric surgery.

  • Glycemic control parameters of female mice with RYGB assessed at different time points after exposure to high-fat diet. Taken together these results indicate a shift towards a more anorexigenic profile in the hypothalamus and suggest an improved sensitivity to endogenous leptin in HFD-fed mice following bariatric surgery.

  • Figure 8.

Previous surgry in Y2R-KO mice are inconsistent. WM mice were given a fixed percent of calories from chow so their data is not shown. No other invasive tests, such as large volume blood sampling, were performed so as not to perturb body weight. Upon induction of lymphedema, HFD-treated mice did not exhibit aggravated oedema and no morphological differences were observed in the blood and lymphatic vasculature. Blood was sampled 6 days later for measurement of blood glucose, plasma LEAP2, and acyl-ghrelin.

Find articles by Mani, B. Thank you for visiting nature. These changes occurred to a greater extent at week 8. Plasma acyl-ghrelin is regulated at least in part by metabolic status.

Background

This article has been cited by other articles in PMC. Article Google Scholar 9. Mice treated with this cytokine lose weight by 'sweating' fat Jul 29, Together, the results discussed so far suggest that the plasma LEAP2 depends on both the long-term, underlying metabolic state e.

  • Adiposity index was defined as fat mass divided by lean mass.

  • Although body length at termination was significantly increased in high-fat fed mice, there were no significant differences between genotypes in either low- or high-fat fed mice Supplementary Figure S2r.

  • Mice were individually housed and sham injected for 5 days with intraperitoneal i. Although coordinated behavioral, dietary, psychological, and medical therapy can be effective in curbing weight gain and disease progression for a while 789long-term effectiveness is rather disappointing 5.

  • Force, U.

  • When RYGB was performed in males of the same age, none gained significant weight after high-fat diet exposure Fig. Experiments on obese mice involved a third group: sham-operated pair-fed mice sham-PF.

Plasma leptin levels were also not significantly higher in RYGB compared to non-surgical chow controls Fig. Statistical analysis was performed using RStudio version 1. In males, the overall effect was similar although the mice in cohort 2 which gained significant body weight on high-fat diet were only partially protected see next paragraph. Jejunal gluconeogenesis associated with insulin resistance level and its evolution after Roux-en-Y gastric bypass. Prevalence of childhood and adult obesity in the United States, —

  • Early reduction of resting energy expenditure and successful weight loss after Roux-en-Y gastric bypass. Figure 7.

  • Both strains fed 12 weeks of HFD-developed obese phenotypes.

  • Times of measurements of food intake FIenergy expenditure and activity in metabolic chambers Mglucose tolerance Gand insulin tolerance I are indicated above the x-axis.

Surhery adipose tissue was also infiltrated by fewer activated T cells and by more anti-inflammatory regulatory T cells. J Lipid Res. Foreign Relations See all channels. Nat Commun. The two male mice with RYGB not resisting weight gain are indicated by the gray and black dots. PLoS One 8e

Cortical and trabecular deterioration in mouse models mouse obesity surgery Roux-en-Y gastric bypass. This makes it difficult to demonstrate the specific effects of the surgery on food intake and body weight. Rubino F. Published : 24 October By assessing candidate mechanisms both before and after exposure to obesogenic and diabetogenic diets or challenges, the separate contributions of direct and indirect effects of the surgery can be distinguished. For sham mice, intestine was cut in fragments reproducing the length of the limbs of DJB mice. Blood glucose was measured using a hand-held glucometer Accu-chek, Roche Diagnostic Corporation, Indianapolis, IN at 0, 15, 30, 60 and min.

Introduction

Exp Diabetes Res. Together, these results highlight that the initial blunting in food intake after DJB could not explain the marked improvement surgery glucose homeostasis at surgerry end of the study period. Steele, K. Background It is well established, in human and rodent models, that caloric excess and the ensuing diet-induced obesity DIOresult in a state of chronic, low-grade inflammation and accumulation of professional immune cells such as macrophages in metabolic tissues including liver, adipose and muscle.

The sham surgical procedure was performed in parallel to RYGB with similar abdominal incision, physical manipulation of stomach and intestine without transection or re-anastomosisand suturing. Surgfry receptor signaling is required for weight loss after gastric bypass surgery. The DJB-induced decrease in food intake was transient and resumed to normal in 7—8 days, suggesting that decreased food intake could not account for the benefits. Insulin sensitivity was also protected in mice with prior RYGB when compared to sham as measured by insulin tolerance at 25 weeks Fig. Sci Rep 9,

In particular, as compared with presurgery levels, VSG reduced plasma LEAP2 levels in the postprandial state, but not the fasted state. Obesity Silver Spring ; 19 — Fisher D. Find articles by He, Z.

Schauer P. Hansen C. Lond ; 36 — Fisher D. The body weight-lowering effect of duodenal bypass surgery over the first 10 days after surgery seen in WT mice was greatly reduced in mice with PYY-deficiency [ 34 ].

Associated Data

When sometimes, fecal lipid loss was studied and evidenced, this was not obesity surgery as a possible important cause of observed body weight loss, perhaps because other processes, e. The DJB-induced decrease in food intake was transient and resumed to normal in 7—8 days, suggesting that decreased food intake could not account for the benefits. The study is published ahead of print in the American Journal of Physiology-Endocrinology and Metabolism. However, other studies have shown that exposure to high-fat diet, and not obesity itself, rapidly induces hypothalamic inflammation Yi et al.

However, it also suggests that these animals continue to defend a higher body weight and would rapidly regain weight if returned to a more obesogenic environment Barbosa-da-Silva et al. We have shown here that two different models of bariatric surgery have an ameliorating effect on HFD-induced hypothalamic metaflammation and microgliosis while normalizing hypothalamic Agrp expression and the anorexigenic response to exogenous leptin, despite ongoing exposure to HFD. Sci Transl Med. Because hypothalamic metaflammation is associated with leptin resistance within the MBH Zhang et al. Lifestyle, diabetes, and cardiovascular risk factors 10 years after bariatric surgery. Charles, MO. Following bariatric surgery, rodents that are induced to gain weight via pharmacologic blockade of central melanocortin receptors, rapidly return to their stable, post-operative body weight after removal of the blockade.

These findings contrasted with those for plasma acyl-ghrelin, which was generally lower in obese states. Obedity YY levels are elevated after gastric bypass surgery. There were no significant differences between any groups in locomotor activity assed in the metabolic chambers at thermoneutrality Figure 4 c. Plasma LEAP2 Figure 3H was higher in diabetic mice, as was plasma acyl-ghrelin Figure 3Iwhich is in line with several previous studies 1438 — 4053 ,

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All 8 female mice with RYGB were completely healthy to the end of the 62 week postsurgical observation period, which is remarkable high fat diet mouse obesity surgery that for the last 50 weeks they almost exclusively consumed a Western-style high-fat diet rich in saturated fats. This was associated with substantial fat malabsorption, since total lipid amount in feces was on average 7 times higher in DJB-WT mice than that in their counterparts sham or sham-PF mice Figure 3 E. This seems consistent with the crucial role of hypothalamic MC4R in the control of energy homeostasis. The energy requirement of the body being positively correlated with body weight, it decreases as body weight decreases. Fecal losses, including excretion of lipids, were studied from the feces recovered in metabolic cages.

Body composition analysis showed that neither surgical procedure resulted in a loss of lean body mass and differences in weight between groups was again almost entirely accounted for by adipose mass, with no statistical difference in lean body mass seen between cohorts Fig. Roux-en-Y gastric bypass enhances energy expenditure and extends lifespan in diet-induced obese rats. Obes Surg 26— In stark contrast, all 8 female mice with RYGB completely resisted weight gain on the high-fat diet up to 50 weeks. It is noteworthy that malabsorption was suggested to correlate with the lipid content of the diet [ 31 ]. Herrick, Kristin M. Hypothalamic proinflammatory lipid accumulation, inflammation, and insulin resistance in rats fed a high-fat diet.

Conclusions: These results indicate that increased adiposity rather than dietary influences determines predisposition to or severity of lymphedema. Both strains fed miuse weeks of HFD-developed obese phenotypes. Glucose and Insulin Tolerance Tests Intraperitoneal glucose tolerance tests were performed at either month 3 or 4 in all cohorts, as indicated. Mice fed standard chow during the entire study duration of 12 weeks served as controls lean group.

Mechanisms responsible for obesity-induced ED are unknown. However, we do not guarantee individual replies due to the high volume of messages. In conclusion, this study suggests that human colon cancer growth is accelerated in animals that are obese and insulin resistant due to the consumption of an HFWD. For general feedback, use the public comments section below please adhere to guidelines.

Roux-en-Y gastric bypass surgery is effective in fibroblast growth factor deficient mice. Nestoridi E. After the start of high fat diet mouse obesity surgery diet, glucose tolerance and insulin tolerance tests were performed at various time points as indicated in the results, and energy expenditure was measured in metabolic chambers at about 6 and 30 weeks after start of high-fat diet. Obesity Silver Spring 23— Loss of insulin resistance after Roux-en-Y gastric bypass surgery: a time course study. However, one of the clearly negative outcomes of this bariatric surgery in both rodents and humans, namely loss of bone mineral density, was not prevented in this model.

Recently, the liver- and small intestine—derived peptide liver-enriched antimicrobial peptide-2 LEAP2 was reported to act as an endogenous antagonist of GHSR Altered secretion and circulating levels of key gut hormones have been proposed as one of the major mechanisms driving metabolic disease resolution following RYGB and other bariatric surgeries [ 910 ]. Mechanisms responsible for obesity-induced ED are unknown. Although the same trend was observed in WT mice, it was not significantly different. Association between bariatric surgery and macrovascular disease outcomes in patients with type 2 diabetes and severe obesity. E Relationship of fasted plasma acyl-ghrelin 0 hours with BMI in cohort 2. Furthermore, concentrations of LEAP2 peptide from an additional source Peptide International; catalog PLPs estimated using the kit matched fairly well the expected concentrations, as determined from adding a known quantity of peptide to a known volume of assay buffer Supplemental Figure 1C.

Find articles by Brandon Boland. In line with this view, the metabolic status of an organism predicts sensitivity to ghrelin, with fasting increasing and obesity decreasing ghrelin sensitivity. From imaging neurons to measuring their true activity 37 minutes ago.

Exaggerated glucagon-like obesity surgery 1 response is important for improved beta-cell function and glucose tolerance after Roux-en-Y gastric bypass in patients with type 2 diabetes. Find articles by Bell, J. Further studies are required to address the potential impact of these VSG surgery— and STZ treatment—associated changes, as compared with the more usual physiological pattern of inverse regulation of LEAP2 and acyl-ghrelin, and in particular, whether this dysregulation could affect overall metabolic responses to endogenous or administered acyl-ghrelin in those settings. The present study was designed to examine the role of enteric glial cells EGCs in colonic neuromuscular dysfunctions in a mouse model of high-fat diet HFD -induced obesity. In weight-matched mice, the amount of food necessary to maintain body weight of the respective RYGB groups was significantly less than food intake of RYGB mice for the first 15 days after surgery, and similar for the two genotypes.

Support Center Support Center. Total fecal losses were about 5 times and lipid losses 7 times higher in DJB-mice than in control obessity and pair-fed mice, and could high fat diet mouse obesity surgery for the weight loss of mice. Effect of roux-en-Y gastric bypass surgery on bile acid metabolism in normal and obese diabetic rats. At termination, trunk blood was collected and tissues were weighed and harvested. The primary therapy for obese patients who have T2DM is to induce weight loss, which improves major parameters involved in the pathogenesis of T2DM [ 2 ].

Interestingly, plasma LEAP2 in obese mice at 12 weeks was higher than the concentrations measured in the same mice at 8 ogesity, indicating that plasma LEAP2 continues to increase with further increases in body weight Figure 1M. The gut hormone response following Roux-en-Y gastric bypass: cross-sectional and prospective study. Support Center Support Center. A postprandial change in plasma LEAP2 also was observed in 2 separate human cohorts.

Published : 04 May Antiobesity and emetic effects of a short-length peptide YY analog and its PEGylated and fay derivatives. View author publications. Also, activity in reward-related brain areas in response to viewing palatable food pictures that was reduced after RYGB was restored with GLP-1 receptor blockade [ 28 ], suggesting that increased GLP-1 receptor signaling to the brain may be important for the decreased appetite and weight loss after RYGB. Trends in obesity among adults in the United States, to

  • Liver microsomal glucosephosphatase is competitively inhibited by the lipid products of phosphatidylinositol 3-kinase. This prevention of obesity model should be crucial for identifying the molecular mechanisms underlying gastric bypass surgery.

  • Suppressing endogenous LEAP2 function in mice with neutralizing antibodies was shown to boost fasting-induced increases in GH release, presumably by enhancing endogenous acyl-ghrelin action

  • Conserved shifts in the gut microbiota due to gastric bypass reduce host weight and adiposity. Quantitative RT-PCR and fluorescent microscopy were used to evaluate hypothalamic inflammatory gene expression and microgliosis.

  • J Sex Med.

  • Cite this article Odom, M. This site uses cookies to assist with navigation, analyse your use of our services, and provide content from third parties.

Conclusions: Reduced white adipose tissue inflammation, modification of adipose tissue development brown vs. In this study, we use two mouse models to surgeru the effects of bariatric surgery on hypothalamic metaflammation. Data obtained from 48 h-studies in WT obese mice placed in metabolic cages showed that food intake, water consumption and urinary volume were similar in sham, DJB and sham-PF mice Figure 3 A—C. In contrast, bariatric surgeries have emerged as an effective treatment for obesity and T2DM [ 34 ]. Boland B. Int J Obes Lond 38— Gut-brain glucose signaling in energy homeostasis.

Hatoum, I. You can also search xurgery this author in PubMed Google Scholar. Roux-en-Y gastric bypass surgery suppresses hepatic gluconeogenesis and increases intestinal gluconeogenesis in a T2DM rat model. Mumphrey, Zheng Hao, R. Fasting plasma leptin was measured only at termination of the experiment from the same trunk blood sample, using the same methods as for fasting plasma insulin.

Leigh Townsend. During a second fasted visit approximately 1 week later, overnight-fasted venous blood samples were taken at approximately pm. Jul 30, They also displayed significantly higher levels of leptin; however, there was a negative correlation between leptin levels and tumor size.

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Zane B. The gut hormones PYY and GLP-1 amide reduce food intake and modulate brain activity in appetite centers in humans. Strauss Professorship in Biomedical Research, the Mr. Three cohorts of mice were used in this study. Endothelininduced responses in isolated mouse vessels: the expression and function of receptor types.

  • All 8 female mice with RYGB were completely healthy to the end of the 62 week postsurgical observation period, which is remarkable considering that for the last 50 weeks they almost exclusively consumed a Western-style high-fat diet rich in saturated fats. Timing of bariatric surgery for severely obese adolescents: a Markov decision-analysis.

  • Sham surgery consisted of laparotomy only, without transection of jejunum and stomach.

  • Herrick, Kristin M.

  • Food intake was measured daily. Also, there was no mortality or complications in any of the mice with RYGB surgery.

None of the 8 fiet and 13 male mice undergoing RYGB at 5—7 weeks of age had any complications during the immediate postoperative period. Both surgical groups maintained a stable, lower body weight, equivalent to CD controls, through the end of the study Fig. Shin A. Homeostatic model assessment of insulin resistance HOMA-IR at 13 weeks was calculated using the baseline measurement of blood glucose obtained during the GTT and the plasma insulin assay results. About this article.

Srgery male mice with prior RYGB both cohorts were combined after finding no significant differences between them the reverse time-dependent effects were observed. DIO-SHAM animals demonstrated significant glucose intolerance with both elevated fasting and glucose area under the curve. Bariatric surgeries result in long-term stable weight loss and improved metabolic function. Results DJB induced a dramatic decrease in body weight and improvement in glucose control glucose- and insulin-tolerance in obese wild type mice fed a high calorie diet, for 25 days after the surgery. Schultes B. The BD-IL model of bariatric surgery, developed to investigate the effects of increased serum bile acids independent of surgical rearrangement of nutrient flow, results in weight loss and a reversal of DIO metabolic dysregulation, similar to that of RYGB Flynn et al.

Abumrad Authors Mary K. Also, there was no mortality or complications in any of the mice with RYGB surgery. Interestingly, this study also shows that despite the beneficial metabolic high fat diet mouse obesity surgery of RYGB surgery, some of its negative sequelae persist; in particular, we show that RYGB decreased bone mineral density in a body weight-independent manner which confirms pervious observations in RYGB operated rats 3540mice 4142and humans Statistical analysis was performed using RStudio version 1. To distinguish the specific effects of DJB surgery from those related to the transient decrease in food intake, a group of sham-operated mice pair-fed with DJB mice was studied sham-PF, grey diamond, Figure 1. American Journal of Physiology-Endocrinology and Metabolism.

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Revised : 01 April Obesity: Pathophysiology and management. Learn More. Andrews Zane B.

Apart from any fair dealing for the obezity of private study or research, no part may be reproduced without the written permission. Study finds reduced risk of cataracts associated with obesity surgery May 13, Learn more. Similarly, therapies that increase plasma LEAP2 might also be beneficial particularly in individuals with obesity who have achieved weight loss, so as to counteract the naturally occurring falls in LEAP2 that otherwise may contribute to rebound weight gain, coincident with increases in plasma ghrelin 69 Use this form if you have come across a typo, inaccuracy or would like to send an edit request for the content on this page.

Although there was a slight reduction in fat mass, lean mass, and adiposity index in mice with RYGB when compared to mice with sham and mice hihh surgery before introduction of the high-fat diet, none of these differences reached significance Fig. In the course of this work, we were led to raise the question of eventual macronutrient malabsorption in DJB-mice. J Neurosci. In stark contrast, all 8 female mice with RYGB completely resisted weight gain on the high-fat diet up to 50 weeks. DIO-Rev animals normalized their glucose tolerance while BD-IL surgical animals showed a significant decrease in both fasting and stimulated glucose levels well below that of CD controls. Indeed, energy expenditure is positively correlated with body weight.

The homogenates were placed in a capillary tube and centrifuged and the length high fat diet mouse obesity surgery the different layers was measured under a microscope. When RYGB was performed in males of the same age, none gained significant weight after high-fat diet exposure Fig. Note reduced growth of mice with RYGB with significant difference in body weight at 10 weeks after surgery compared to all other groups. Simerly 3Naji N. Insulin sensitivity was also protected in mice with prior RYGB when compared to sham as measured by insulin tolerance at 25 weeks Fig. Results: In SG mice, food intake was reduced transiently, but weight loss was significant and persistent compared to controls SG vs. Stylopoulos, N.

User comments. Jorgensen N. Acyl-ghrelin administration increases food intake, body nouse gain, and blood glucose 1211 Since integrity of vagal afferents innervating the small intestine through the celiac branches are partially required for the beneficial effects of RYGB in rats [ 46 ], a case for Y2R on vagal afferents could be made. References 1.

Jejunal gluconeogenesis associated with insulin resistance level and its evolution after Roux-en-Y gastric bypass. Glycemic control parameters of female mice with RYGB assessed at different time points after exposure to high-fat diet. Duodenal-jejunal bypass surgery was performed under controlled anesthesia as described in details previously [ 30 ]. Bariatric surgery is increasingly considered in severely obese adolescents and even children and an intensive debate of pros and cons has begun 79101112131415 Mol Metab 5— Quantitative RT-PCR and fluorescent microscopy were used to evaluate hypothalamic inflammatory gene expression and microgliosis. However, a weak energy malabsorption, very variable according to patient and of lesser importance in front of the decrease in food intake, was suggested to be mainly dependent on the patient's diet [ 31 ].

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Obesity is associated with hypothalamic injury in rodents and humans. However, studies have found that men obesit women do not respond the same way to weight loss interventions. As a consequence, weight loss ceases once the energy requirement of the body decreased is fulfilled by the amount of food ingested decreased in the case of caloric restriction, malabsorbed here. You can also search for this author in PubMed Google Scholar. Given the putative importance of IGN in RYGB, the initial aim of this work was thus to assess its causal role in the metabolic benefits deriving from this surgery.

DIO-Rev animals normalized their glucose tolerance while BD-IL surgical animals showed a significant decrease in both fasting and stimulated glucose levels well below that obesit CD controls. Discussion With obesity and metabolic diseases increasingly afflicting children and adolescents 12it will be important to investigate long-term effects of therapeutic approaches for prevention and treatment. For the first time we demonstrate that RYGB at an early age completely prevents diet-induced obesity later in life in female mice and prevents obesity in a majority but not all male mice. Stylopoulos, N.

It appears that you are currently using Ad Mouse obesity surgery software. Figure 5 Changes associated with food intake in humans. High-fat diet associated with obesity induces impairment of mouse obesiity cavernosum responses. Our data suggest that blood glucose may itself regulate plasma LEAP2, independently of body mass or feeding status. Blood glucose was determined at 0, 15, 30, 60,and min for cohort 1; 0, 15, 30, 60, and min for cohort 2; and 0, 15, 30, 60, 90, and min for cohort 3.

J Clin Endocrinol Metab— Bariatric diet mouse obesity versus conventional medical therapy for type 2 diabetes. J Pediatr Surg 51— Design and conception of studies H. One mechanism proposed and documented by several groups to account for the metabolic benefits of RYGB relates to the activation of intestinal gluconeogenesis IGN [ [21][22][23][24] ]. Image modified from Albaugh et al.

J Am Diet Assoc. Women: Lower-fat Diet Key to Liver Health Following Weight-loss Surgery Study in mice finds liver fat levels still high in females who continued high-fat diet after gastrectomy. Face to these intriguing results, we were led to raise the question of an eventual fecal loss of energy to account for the prolonged body weight loss in absence of prolonged decrease in food intake and its maintenance during food recovery, as observed in ours [ 30 ] and others [ [9][10][11][12][13][14]1617192039 ] studies. Search Search articles by subject, keyword or author. In addition, a key feature of RYGB is the rapid disgusting for high calorie food in both human patients and laboratory rodents [ [45][46][47] ]. Nestoridi E. In both female and male mice liver fat content as visualized with oil-red-O staining was greatly increased in both obese groups sham and non-surgical high-fat fed controls compared to non-surgical chow mice Fig.

For sham mice, intestine was cut in fragments reproducing the length of the limbs of DJB mice. All 8 female mice with RYGB were completely healthy to the end of the 62 week postsurgical observation period, which is remarkable considering that for the last 50 weeks they almost exclusively consumed a Western-style high-fat diet rich in saturated fats. Penhoat A.

Mice fed standard chow during the entire study duration of 12 weeks served as controls lean group. Starting at about 6 petbox acquired hypothyroidism of age, some rat were exposed to HF diet or a two-choice diet consisting of HF and LF for the duration of the experiment, except for periods in the metabolic chamber when they were exposed to only HF diet. Changes associated with obesity in fasted humans. Naveilhan P. There were no differences in total energy intake between any groups before surgery Figure 3 a,b.

Roux-en-Y gastric bypass enhances energy expenditure and extends lifespan in diet-induced obese rats. Mumphrey View author publications. Given the known effects of surgery metaflammation on hypothalamic leptin resistance, these studies raise the possibility that bariatric surgery may improve hypothalamic metaflammation contributing to a lower set point of defended body weight. Publication types Research Support, Non-U. The lower end of the cut jejunum was anastomosed to the small gastric pouch and the upper end of the cut jejunum was anastomosed to the side of the lower jejunum.

Discussion Given the strong anorexigenic and body weight lowering effects of PYY and Y2R-agonists [ 203233373839 ], it hiigh be expected that Y2R-deficient mice eat more and gain more weight than WT mice. Specifically, postprandial circulating diet mouse obesity of both GLP-1 and PYY show a rapid and sustained increase after both RYGB and vertical sleeve gastrectomy VSG in humans [ 11121314151617 ], as well as in rodent models [ 1819 ]. Interestingly, plasma LEAP2 in obese mice at 12 weeks was higher than the concentrations measured in the same mice at 8 weeks, indicating that plasma LEAP2 continues to increase with further increases in body weight Figure 1M. Version 1 August 19, : Electronic publication Version 2 September 3, : Print issue publication.

Results: In SG mice, food intake was reduced sugery, but weight loss was significant and persistent compared to controls SG vs. Close banner Close. Glucose and insulin tolerance tests Glucose tolerance test GTT and insulin tolerance test ITT were carried out 2 and 3 weeks after the surgery, respectively. It will also be important to further analyze the molecular mechanisms leading to these changes in energy intake and expenditure that render protection.

In peripheral tissues, metaflammation has been linked to metabolic dysfunction including insulin resistance Hotamisligil et al. Duodenal-jejunal bypass induces gluconeogenic capacity in gut limbs receiving nutrients in obese WT mice Face to the intriguing data above, we checked whether the modifications in gluconeogenic capacity took place in mice with DJB as previously observed in rats [ 212224 ] or mice with gastroenteroanastomosis [ 23 ]. Effects of Roux-en-Y gastric bypass on energy and glucose homeostasis are preserved in two mouse models of functional glucagon-like peptide-1 deficiency. American Journal of Clinical Nutrition. It will also be important to further analyze the molecular mechanisms leading to these changes in energy intake and expenditure that render protection. These changes in G6Pase enzymatic activity were associated with marked hypertrophy in both nutrient-receiving limbs after DJB, which is a well-known effect of gastric bypass surgery [ 36 ] Supplemental figure 1bshowing the weight of the 3 limbs reported to length and compared with the corresponding parts in sham- and sham-PF mice.

Therefore, the mechanisms remained elusive. Stroud, A. Publication types Research Support, Non-U. Femurs were dissected at the end of the experiment and bone mineral density BMD was assessed on isolated femurs with micro computed tomography 34 ,

A review of GLP-1 receptor agonists: Evolution and advancement, through the lens of randomised controlled trials. Chow preference was calculated as the percentage of total food intake in kcals obtained from regular chow diet. Andrews Zane B.

Slides were digitally scanned using a NanoZoomer 2. JAMA— Improved rodent maternal metabolism but reduced intrauterine growth after vertical sleeve gastrectomy. Energy expenditure, RER, and locomotor activity were assessed in metabolic chambers at two time points after introduction of the obesogenic diet.

However, a comprehensive study involving mokse mouse strains and a high number of dietary regimen of various compositions in macronutrients has recently established that assimilated lipid is the main driver of body weight gain [ 42 ]. Author information Article surgfry Copyright and License information Disclaimer. Irrespective of the substantial lipid loss around 40 mg per day, see Figure 3 Eit is noteworthy that the cumulative amount of excess stool in DJB-mice compared to sham- or sham-PF mice around mg per day, see Figure 3 D globally matches the weight loss observed during the 25 days of the study around 12 g, refer to paragraph 3. A better understanding of the mechanisms behind these effects may provide insight into novel therapies for obesity. Vitamin D status in growing dairy goats and sheep: Influence of ultraviolet B radiation on bone metabolism and calcium homeostasis. As expected, female mice without surgery or with sham surgery responded promptly to exposure of the two-choice high-fat diet by rapidly gaining body weight and fat mass Fig.

Conclusions: PYY signaling through Y2R is not required for the normal appetite-suppressing and body weight-lowering effects of Obesify in this global knockout mouse model. Keywords: mouse obesity motor dysfunction; enteric glia; inflammation; obesity; substance P. Furthermore, concentrations of LEAP2 peptide from an additional source Peptide International; catalog PLPs estimated using the kit matched fairly well the expected concentrations, as determined from adding a known quantity of peptide to a known volume of assay buffer Supplemental Figure 1C.

Here, we tested the hypothesis that LEAP2, similar to acyl-ghrelin, represents a metabolic hormone that is regulated by obessity mass, feeding, and blood glucose and that works in concert with acyl-ghrelin to modulate GHSR activity as a response to those metabolic changes. Sign up for email alerts. Lond ; 36 — For general inquiries, please use our contact form.

Associated High fat diet mouse obesity surgery Supplementary Materials nutrientss Weight loss, appetite suppression, and changes in fasting and postprandial ghrelin and peptide-YY levels after Roux-en-Y gastric bypass and sleeve gastrectomy: a prospective, double blind study. Cite this article Odom, M. Meal-induced hormone responses in a rat model of Roux-en-Y gastric bypass surgery. Here, we report clinically relevant findings regarding changes in plasma LEAP2 in states of altered energy balance and metabolism.

In contrast, mice with sham surgery of both genotypes had only a small and transient reduction and then continued to gain body weight. Find articles by Michael B. Associated Data Supplementary Materials nutrientss Heydemann A.

Send Feedback. Keywords: obesity, diabetes, body weight, body composition, glucose tolerance, insulin tolerance, incretin, energy expenditure. Hugh the six-hour fasting blood glucose values in petbox acquired hypothyroidism cohorts and the blood glucose values indicated for the intraperitoneal glucose tolerance tests GTTs and insulin tolerance tests ITTsa glucometer was used Ascensia Breeze, Bayer, Germany with tail-vein blood. Blood was sampled 6 days later for measurement of blood glucose, plasma LEAP2, and acyl-ghrelin. Download references. During a second fasted visit approximately 1 week later, overnight-fasted venous blood samples were taken at approximately pm. Methods: Here we assessed the role of Y2R signaling in the response to low- and high-fat diets and its role in the effects of Roux-en-Y gastric bypass RYGB surgery on body weight, body composition, food intake, energy expenditure and glucose handling, in global Y2R-deficient Y2RKO and wildtype WT mice made obese on high-fat diet.

Mice were fasted for 6 h and injected intraperitoneally with high fat diet mouse obesity surgery. Or to: Anthony P. During an initial postprandial visit, they arrived after eating a normal breakfast at home and baseline venous blood was sampled at approximately — pm approximately 5 to 6 hours after breakfast. Jorgensen N. References 1. LEAP2 is synthesized as a 77—amino acid prohormone in humans 76 amino acids in mice 42 that subsequently is processed to its mature form consisting of 40 amino acids with 2 disulfide bridges spanning 4 highly conserved cysteine residues 41 Hao Z.

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